Nutrient TTC + Malt Extract Dipslides (Box of 10)

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Includes TTC
Red Spot Dye

Our BTM2 Dipslide tests for / shows growth of moulds and yeasts on one side and total bacteria on the other side. It can be used to test/monitor the levels of fungi, aerobic bacteria and yeast.

Each dipslide box includes 10 dipslide tests.

Pack Size

10 dipslides

£11.96

	Made in the UK
	Manufactured in ISO 9001 certified conditions.
	Pay via Credit/Debit Card, PayPal or bank transfer online. PO number can be added during checkout if required.

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Volume Discounts
Order 25 or more boxes	Receive a 2.5% discount	Price per box £11.66
Order 50 or more boxes	Receive a 5.27% discount	Price per box £11.33
Order 100 or more boxes	Receive a 8.92% discount	Price per box £10.89
Order 250 or more boxes	Receive a 14.24% discount	Price per box £10.26
Order 500 or more boxes	Receive a 18.89% discount	Price per box £9.70
Order 1000 or more boxes	Receive a 33.65% discount	Price per box £7.94

Product Information
Front Agar Type	Nutrient TTC
Front Agar Colour	Straw
Front Agar Growth	Total Count (TTC / Red Spot)
Rear Agar Type	Malt Extract
Rear Agar Colour	Brown
Rear Agar Growth	Yeasts & Moulds
Shelf Life	6 to 8 months
Certification	ISO 9001
Suggested Incubation Times	24 - 120 Hours
Suggested Incubation Temp	30°C

About the nutrient ttc + malt extract dipslides (box of 10)

The BTM2 Dipslide will grow / detect total count (aerobic bacteria / microbes) on one side and moulds and yeasts on the other side. It can be used to test/monitor the levels of mould, aerobic bacteria and yeast. If there are microbiological contaminants in your sample, they will grow as colonies on the dipslide agar (culture media). The agars on this dipslide are Nutrient Agar with TTC and Malt Extract Agar.

Nutrient Agar is a general purpose agar that tests for a total bacteria count (TVC). The Nutrient agar includes TTC (red spot dye). This means that most colonies growing on the dipslide agar will appear as red spots which makes it easier to interpret results. Malt Extract Agar tests for yeasts and moulds. This is a general purpose slide that can be used in a variety of applications.

You can either dip the slide into your sample, swab onto the dipslide or press the slide against a surface. Once your sample has been transferred onto the dipslide, you then incubate the dipslide. After incubation, you can then count the colonies / spots and compare with the chart provided. A step by step pictorial guide can be found below.

These dipslides come in a pack size of 10 slides per box. The slide has a flexible paddle and is within a shatterproof container. Compatible dipslide incubators can be found here: Dipslide Incubators.

How to use the BTM2 dipslide

Simply remove the dipslide and immerse in the sample for approx 10 seconds. Remove dipslide and allow to drain for a few seconds, then replace the slide back into the tube and incubate for a minimum of 24 hours (up to a maximum of 120 hours).

A comparison chart is included so you can easily compare your results.

Remove Nutrient + Malt Extract slide from tube

Step 1 : Remove slide

Carefully remove the sterile Nutrient + Malt Extract dip slide from its tube ensuring that no contact is made with the agar (to avoid contamination).

Step 2 : If Surface Testing

For surface testing, press the Nutrient + Malt Extract dipslide onto the surface. If needed, place a finger on the bottom of the slide (on the plastic, not the agar) to apply extra pressure.

Step 2 : If liquid testing

For liquid testing, immerse the Nutrient + Malt Extract dipslide into the fluid for 10 seconds then remove and allow to drain for a few seconds.

Step 2 : If swab testing

Apply a sample of the test substance to a sterile swab and gently apply to the agars.

Step 3 : Incubate Slide

Label the tube with the included stickers then place the slide into an incubator and allow to incubate.

Step 4 : Compare results

Count the amount of colonies that have grown on the slide and compare them to the comparison chart provided.

BTM2 Dipslide Features

Flexible Paddle

Each dipslide features a virtually unbreakable and flexible paddle allowing for even, full surface testing.

Easy Run Off Design

Due to the design of the dipslide, fluids will drain off quickly during testing.

Recessed Handle

Each slide has a handle built into the lid that is recessed to help keep your fingers dry during testing.

Side Indicator

One side of the slide will have a circle indicator so you know which side was used for testing which type of contaminant.

Dual Sided

Each dipslide is dual sided to allow multiple contamination testing with one test.

Thick Reliable Agar

Each agar is thick (approx 4mm) and raised above the dipslide to allow for easy surface testing.

What is a BTM2 Dipslide?

The BTM2 dipslide is a dual sided slide coated in two different agars. It features a Nutrient agar to detect total aerobic bacteria and a Malt Extract agar to detect Yeasts and moulds. It is used in multiple industries and is often used to test liquids for contamination (CFU/ml).

Industry sectors

Commonly used to detect 'fuel bug' (microscopic fungi, yeast and bacteria)
Cooling tower waters
Boiler feed water
Engineering and metal cutting fluid contamination testing
General water testing
Ground source heat pump systems / closed loop heating systems

Dip Slide Storage

Store dip slides in a cool dry place (optimum temperature 8-15 degrees c). Use before the expiry date on the box.

Dip Slide Disposal

Used dip slides should be incinerated, autoclaved or soaked in disinfectant for several hours before disposal.

Reading the results

	Nutrient Side
Level of infection in liquids (CFU/ml)	10²	10³	10⁴	10⁵	10⁶	10⁷
Approx colonies per agar	2	12	60	200	500	600+
Example Image
Level of infection on surfaces (CFU/cm2)	0.4	2.5	12	40	100	250

	Malt Extract Side
Level of yeasts in liquids (CFU/ml)	10²	10³	10⁴	10⁵	10⁶	10⁷
Approx yeasts colonies per agar	2	12	60	200	500	600+
Example Image
Level of yeasts on surfaces (CFU/cm2)	0.4	2.5	12	40	100	250

	Malt Extract Side
Level of moulds	Light	Medium	Heavy
Example Image

Customer Questions

Q	Do we require to use an incubator for these slides?
A	Yes. We recommend the use of an incubator as this gives a constant temperature of 30 degrees c and will give more accurate results.
Q	Can the dipslides be used without an incubator? I don't want to have to purchase an incubator because I can't afford it, and I am only wanting to test the creams and lotions I make for my own use. If I left the dipslides in a warm room for several days, would that ultimately give me an accurate reading? I don't mind having to wait several days.
A	Dipslides will need to be kept at a constant correct temperature to provide accurate results. As such, we only recommend using an incubator that can be configured to meet this requirement / the temperature required for the dipslide.
Q	Dipslide instructions indicate 24 to 48 hr incubation. What do I tell the customer if they read after 24 hrs and there is no growth and then there is some in 48 hrs and more in 72 hrs. They want to go with the 24 hour rdg and we are in conflict-I say if slow growers show up in 72 hrs than the microbes are there and the systems should be treated.
A	The nutrient side should start to show bacterial growth after 24 hours (assuming an incubator is used). After 48 hours, you can take your final reading. If incubating after 48 hours, it is important to note if any further growth is "new" growth or "increased growth". e.g. additional red spots or simply larger red spots. We would always suggest waiting the full 48 hours to make sure that the bacteria has grown enough so it is visible to the human eye in 'red dot form'. Of course, if the slide is heavily infected after 24 hours, it's pretty safe to assume after 48, it will be worse and as such, action can be taken (e.g. another test to confirm the results or action taken in line with your risk assessment). The Malt extract side can take a lot longer (up to 120 hours) due to the time it takes for moulds/yeasts to grow.
Q	Hi, I want to know what is limit for Fungal Growth. Your literature says Mold can be identified only as Light Medium and Heavy so what would be acceptable limits for garments of Food Processing Industry.In case of TBC/TTC no. of Colonies can be counted through dots and ETSA has a limit of 20 spots for same.
A	I'm afraid this is not something we could advise on. You would need to speak to the appropriate regulator regarding this.
Q	Hi, I am currently using the product shown in the link below, but is this the best comparative product that you supply.
A	Hello, Yes, the dipslides in the link you provided use Nutrient + tcc and Malt Extract (which is the same as out own Nutrient + Malt Extract Dipslides (TTC = Red spot dye). Hope this helps.