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About the leather dipslide tests (box of 10)
These Leather Dipslides will test for bacteria, yeasts and moulds (fungi). If your test sample contains microbiological contaminants, colonies will grow on the dipslide agar (culture medium). You can then count the colonies (red spots) on the dipslide and compare with the chart provided to assess the level of contamination.
Why are dipslides useful for testing during leather production?
Due to the tendency for spoilage when hides / skins are in the beamhouse ready for tanning, bactericides / fungicides are often used to prevent the skins from becoming putrefied. Dipslides can be used to test the effectiveness of the biocides.
Spoilage can occur when the raw hide initially starts to break down. This is due to micro-organisms that inevitably begin to colonise the skin. Because of this, there are methods to preserve the skin before they reach the tannery (chilling, salting, drying).
Skins are re-hydrated by the soaking process and as bacteria tends to thrive in water, this can be an issue. As well as this, liquids used in later stages can also encourage the growth of micro-organisms. In fact, water is used multiple times throughout the pre-tanning process. Despite all of the precautions taken, as you can see above, the leather has many processes to go through where bacterial growth can be present.
After everything that the leather goes through before it is ready to be used as an end product, it is prudent to test the microbiological status of the leather throughout. This is to ensure that the precautions you put in place are working, that spoilage is avoided and to stop any contaminants affecting workers. Fungal species have been known to cause respiratory issues in tannery workers and bacterial infections can also occur.
In order to get the best results from our dipslides, you will need to use an incubator. You can purchase these from our incubator section.
Nutrient Agar with TTC* and Leather Agar; Leather Agar is a growth medium for yeasts and moulds, which reduces bacterial growth. High levels of bacteria can overwhelm the suppression. This slide offers a total count on one side and Yeasts and Moulds on the other.
How to test leather using LBTM2 dipslides
For testing leather, you may wish to test the surface directly or use swabs.
When using dipslides, you may choose to test different areas of the hide / skins. For example, you could test by swabbing the 'flesh' side of the hide or by swabbing 'hair' before the liming process. This all depends at what point in the pre- tannning process you are testing at and which parts you wish to analyse.
Using a swab, you can follow these instructions:
- Carefully use a sterile swab to collect a sample from the raw hide / skin.
- Apply the swab to the dipslide (on both sides).
- Place the slide back in its container and replace the cap.
- Incubate the slide for 24 - 48 hours.
- Compare your results with the included chart.
Step 1 : Remove slide
Carefully remove the sterile Leather dip slide from its tube ensuring that no contact is made with the agar (to avoid contamination).
Step 2 : If Surface Testing
For surface testing, press the Leather dipslide onto the surface. If needed, place a finger on the bottom of the slide (on the plastic, not the agar) to apply extra pressure.
Step 2 : If liquid testing
For liquid testing, immerse the Leather dipslide into the fluid for 10 seconds then remove and allow to drain for a few seconds.
Step 2 : If swab testing
Apply a sample of the test substance to a sterile swab and gently apply to the agars.
Step 3 : Incubate Slide
Label the tube with the included stickers then place the slide into an incubator and allow to incubate.
Step 4 : Compare results
Count the amount of colonies that have grown on the slide and compare them to the comparison chart provided.
Leather Dipslide Features
Each dipslide features a virtually unbreakable and flexible paddle allowing for even, full surface testing.
Easy Run Off Design
Due to the design of the dipslide, fluids will drain off quickly during testing.
Each slide has a handle built into the lid that is recessed to help keep your fingers dry during testing.
One side of the slide will have a circle indicator so you know which side was used for testing which type of contaminant.
Each dipslide is dual sided to allow multiple contamination testing with one test.
Thick Reliable Agar
Each agar is thick (approx 4mm) and raised above the dipslide to allow for easy surface testing.
Product Reference : LBTM2
Internal catalogue reference : DS001-38
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