E.coli Test Kit
  • E.coli Test Kit
  • E.coli Test Kit
  • E.coli Test Kit

E.coli Test Kit

Escherichia coli kit includes 2 x boxes of dipslides, incubator (up to 2 dipslide capacity) and UV lamp for identifying e.coli colonies.

£177.13 Tax excluded

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Product specification

This kit will enable you to test for and identify strains of escherichia coli.
Please note, these dipslides also test for total plate count and coliforms. You could use them to assess general microbiological contamination and then use the UV lamp included in the kit to presumptively ID e.coli.

Included in the kit:

E.coli Dipslides (2 boxes)

These dipslides are dual sided with Plate Count Agar (total count) on one side and MacConkey3 Agar (coliforms) on the other side. The MacConkey3 agar has been formulated with the addition of MUG reagent. MUG is what makes it possible to view e.coli colonies under longwave ultraviolet light.

E.coli produces the enzyme ß-glucuronidase. This enzyme is produced by over 95% of e. coli strains.* MUG reagent (4-methylumbelliferyl-ß-D-glucuronide) reacts to the production of the enzyme ß-glucuronidase by breaking down and releasing 4-methylumbelliferone. This reaction produces a green/blue fluorescence that can be viewed under longwave ultraviolet light.

Pink colonies that fluoresce under UV light can be presumptively identified as e.coli colonies. Colonies that do not fluoresce indicate a negative result. Colonies that are not pink that fluoresce also indicate a negative result.

GNAT MX2 Incubator (35 degrees C model)

The MX2 incubator is a compact unit that will incubate up to 2 dipslides simultaneously. The incubator is pre set to 35 degrees C and is ready to use. The incubator will just need to be allowed to stabilise for 20 minutes before use. The MX2 has a heat reservoir that helps maintain a constant temperature. An accurate result cannot be guranteed if an incubator isn't used.

The MX2 runs from 12 Volts DC and has overload protection. The incubator comes with a mains power pack (110-240v AC) and has compatible plug adapters for UK, USA, Australia and Europe.
Dimensions: 15 x 8 x 9 cm

Longwave UV lamp

In order to view e.coli colonies on the MacConkey3 MUG agar, you will need to use a Longwave 366nm wavelength UV lamp like the one in this kit.

This is a 4W handheld lamp that requires 4 x AA batteries (not included).

For best results, examine the dipslides using the UV light in the dark.

E-coli dipslide kit instructions

Fluid sample testing

1. Remove the dipslide from its container. Be careful not to touch the agar while doing this.

2. Dip the dipslide in the sample for approximately 10 seconds.

3. Allow the the fluid to drain from the dipslide.

4. Place the dipslide back within the container. Seal firmly.

5. Label the dipslide using the included labels.

6. Place the dipslide within the incubator for 24-48 hours at 35 degrees C.

7. Once incubated, compare the amount of colonies on the dipslide with the chart included. The straw coloured (plate count) side will indicate how many viable organisms are within your sample. The red (MacConkey 3) side will indicate the level of coliforms in your sample. Red / pink colonies on the MacConkey 3 side indicate coliforms. The chart will help you assess the level of total count / coliform contamination.

8. To check for e-coli colonies, examine the MacConkey 3 side under the uv lamp. Turn the light out / examine in the dark so that it is easier to view.

9. Red / pink colonies that fluoresce blue / green under UV light can be presumptively identified as e.coli colonies. Colonies that do not fluoresce indicate a negative result. Colonies that are not red / pink that fluoresce also indicate a negative result.

Surface testing

1. Remove the dipslide from its container. Be careful not to touch the agar while doing this.

2. Holding the tabs at both ends, press one side of the dipslide onto the surface that you are testing. Press down gently ensuring full contact with the surface.

3. Repeat on the other side of the dipslide.

4. Place the dipslide back within the container. Seal firmly.

5. Label the dipslide using the included labels.

6. Place the dipslide within the incubator for 24-48 hours at 35 degrees C.

7. Once incubated, compare the amount of colonies on the dipslide with the chart included. The straw coloured (plate count) side will indicate how many viable organisms are within your sample. The red (MacConkey 3)side will indicate the level of coliforms in your sample. Red / pink colonies on the MacConkey 3 side indicate coliforms. The chart will help you assess the level of total count / coliform contamination.

8. To check for e-coli colonies, examine the MacConkey 3 side under the uv lamp. Turn the light out / examine in the dark so that it is easier to view.

9. Red / pink colonies that fluoresce blue / green under UV light can be presumptively identified as e.coli colonies. Colonies that do not fluoresce indicate a negative result. Colonies that are not red / pink that fluoresce also indicate a negative result.

Reference

*https://www.fda.gov/food/foodscienceresearch/laboratorymethods/ucm064948.htm

References

https://www.fda.gov/food/foodscienceresearch/laboratorymethods/ucm064948.htm

Ecoli Kit / SCM

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