Legionella Test Kit (Rapid 25 minute test) 100 CFU / L

  • Hydrosense one ultra Legionella test
  • Legionella water Test
  • Legionella Test
  • Legionella test kit
  • Positive Legionella Test
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Hydrosense one ultra Legionella test
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Legionella test kit - rapid test, get results in 25 minutes. Very sensitive test - 100 CFU / L. Easy to read results - 1 line = indicates negative, 2 lines = indicates positive.

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Product Information

About Our legionella test kit (rapid 25 minute test) 100 cfu / l

Legionella test kit - sensitivity 100 CFU/ L (Hydrosense One Ultra)

Simple and easy to use Legionella test for onsite testing. No need to transport samples.
Detects Legionella pneumophila sg1 antigen - this species is responsible for over 90% of Legionnaires' disease cases.
No specialist training required.
Rapid test - results in 25 minutes. Fastest legionella test available.
Easy to decipher lateral flow test. 2 lines = indicates positive result. 1 line = indicates negative result.
Has a recovery rate of 80% (lab culture test recovery rate is approx / on average 64%).
Sensitive test - offers 100 CFU / L detection levels for water tests.
Water samples can be used from any source.

Legionella Water testing kit includes:

  • 1 x 250 ml beaker
  • 1 x 60 ml syringe
  • 1 x Filter
  • 1 x 1ml syringe with buffer
  • 1 x lateral flow Legionella test
  • Full instructions (with images)

How to test for Legionella using the rapid test kit

Legionella water testing steps are shown below. Full instructions are also included with the testing kit.

Step 1 : Collect water sample

Step 1 : Collect water sample.

Collect 250 ml water using the included beaker.

Step 2 : Draw water sample into syringe.

Step 2 : Draw sample into syringe.

Draw the sample up into the 60ml syringe to the 50 - 60ml mark.

Step 3 : Attach filter to syringe

Step 3 : Attach filter to syringe.

The sample will then be passed through the hollow fibre filter. Tighten the end cap of the filter before use. Attach the filter to the end of the syringe

Step 4 : Squeeze the liquid from the syringe into the filter

Step 4 : Squeeze the liquid from the syringe into the filter.

Squeeze the liquid from the syringe through into the filter. Hold the filter over a container / sink while doing this so it catches the water. Repeat this until the 250ml has all been filtered.

Step 5 : Move filter cap to the bottom.

Step 5 : Move filter cap to the bottom.

Remove the cap from the top of the filter, screw it onto the bottom of the filter.

Step 6 : Attach 0.25ml legionella recovery buffer syringe to filter.

Step 6 : Attach 0.25ml recovery buffer syringe to filter.

Next use the syringe containing 0.25ml of recovery buffer to extract bacteria from the filter. Remove the red cap on the 1ml syringe and keeping the filter upright, attach the syringe to the top of the filter then invert the filter, keeping it vertical with the syringe on the bottom and filter on the top.

Step 7 : Extract the concentrated sample from the filter.

Step 7 : Extract the concentrated sample from the filter.

This step extracts the concentrated sample from the filter. Pull the syringe back to the 0.5ml mark then push the liquid buffer into the filter then pull back to 0.5ml again and then push in and repeat 3 times.

Step 8 : Collect 0.1ml of the sample.

Step 8 : Collect 0.1ml of the sample.

Draw the syringe back to the 0.5ml mark, push the syringe in slowly until you reach the 0.1ml mark. If this creates an air bubble, try again by pulling the syringe back and pushing it out.

Step 9 : Remove the Legionella test strip from its packaging.

Step 9 : Remove the Legionella test strip from its packaging.

Take the test strip out of its packaging. Only do this at this stage and not before.

Step 10 : Remove the filter and add the test sample to the legionella test strip.

Step 10 : Remove the filter and add the test sample to the Legionella test strip.

Place the test on a flat surface. Detach the syringe from the filter by twisting from the filter then add the 0.1ml sample to the sample port on the test.

Step 11 : Wait 25 minutes then read the result.

Step 11 : Wait 25 minutes then read the result.

Start a timer for 25 minutes. Read the Legionella test after 25 minutes. Two lines = Legionella detected.

Legionella water testing video instructions


Recommended for legionella monitoring in many applications such as:

  • Cooling water - cooling towers / HVAC systems
  • Water tanks
  • Water fountains
  • Showers / taps
  • Car wash installations
  • Sprinkler systems

Examples of settings / facilities that benefit from regular testing:

  • Hospitals
  • Care Homes
  • Rental accommodation
  • Hotels
  • Leisure centres
  • Dental surgeries / Dental practices

Please note that testing regularity / type of testing will be dependent on regulations / H & S guidelines regarding your specific industry or location.

Customer Questions


Are there special storage recommendations for these Legionella tests?


The tests should be stored at room temperature, preferably below 30°C (86°F).


How should the Legionella tests be disposed of?


Used Legionella tests can be disposed of in normal waste procedures. The instruction leaflet that you receive with your test will include a full list of components that can and cannot be recycled.


What if the test result is negative?


A negative test result on the Legionella test indicates that the Legionella pneumophila SG1 antigen has not been detected in your sample or that the levels were below the limit of detection.


Do I need to do a lab test?


Studies show that in lab culture tests, recovery rates are not very high (approx 64%) which means that false negative results using the lab culture method can happen in up to 35 out of 100 tests. Lab culture testing is also a lengthly procedure compared to the rapid results that are achieved with this test. However, some regulators require that a lab test is conducted, in this case, you should always do so. You can use both methods as this test will alert you quickly as to whether you have a Legionella problem while you are still waiting for lab culture results giving you more peace of mind. Many users will use a rapid test for early notification of an infected sample alongside lab culture or PCR testing.


Can these tests be used in medical applications (e.g urine test for patients)?


No, these are not a medical test. The test is appropriate for testing liquids such as water and is not for medical diagnosis.


How often should I test for Legionella?


This depends on the water source you are testing. For example, HSE state "It depends on the system that you have and the outcome of your risk assessment. For open systems, such as cooling towers, evaporative condensers and spa pools etc, routine testing should be carried out at least quarterly. However, there may be circumstances where more frequent sampling may be required". Always check local regulations in regards to requirements for Legionella testing.


Can a plumber check for legionella?


Yes, The Legionella test kit (LG25-100) allows anyone to conduct a legionella test of a water sample without any prior training.


I'm a landlord (3 properties) and I need to perform legionella risk assessments. Can I use this kit to perform tests to help show compliance?


Landlord legionella risk assessments require monitoring of your water to ensure the health and safety of your tenant. This involves various measures such as flushing a system prior to rental and setting control parameters (e.g. setting the hot water temperature to 60°C). Many landlords use our LG25-100 Legionella test to test the system once all other required measures have been taken. This is then recorded so it can be provided if required in the future.

Additional Details

Product Reference : LG25-100

Internal catalogue reference : DS001-206

Product external rating on reviews.co.uk:

5.0 out of 5 based on 4 customer ratings

Reviews (independently collected by reviews.co.uk)